Šimunić, P. et al.
EQOL (2009) 19-24
THE EFFECT OF AGEING ON CONTRACTION TIME OF
POSTURAL AND NON-POSTURAL SKELETAL
MUSCLES IN MASTER ATHLETES
Boštjan Šimunič*, Rado Pišot*, and Jörn Rittweger**
* Institute for Kinesiology Research, Science and Research centre of Primorska, University of Primorska,
Koper, Slovenia
** Institute for Biomedical Research into Human Movement and Health, Manchester Metropolitan
University, UK
Abstract
Normal aging is characterized by muscular atrophy and a loss of force-generating capacity. The goal of
this research was to investigate the age and sport activity type on postural (vastus lateralis) and non
postural (biceps femoris) muscles’ contraction time in 170 master athletes (athletic events) and 51 non
athletes. We found significant age effect in postural muscle (P = 0.032) and also in non postural muscle
(P < 0.001). However, we found sport activity type effect significant just in non postural muscle (P <
0.001). The interaction effect of age * sport was significant in both observed muscles. Sprinters/jumpers
deteriorate with age the most in postural muscle, while non athletes in non postural muscle. We could
conclude that non postural muscle deteriorate the most without regular sport activity, while postural
muscle have enough daily stimuli to be prevented from major deterioration.
Keywords: Ageing, Skeletal muscle, Sport Activity, Tensiomyography, Athletics, Contraction time
Introduction
Regular physical activity and healthy sports are essential for our quality of life, especially in older age.
Appropriate physical activity and sports for all constitute one of the major components of a healthy
lifestyle, along with healthy diet, tobacco free life and avoidance of other substances harmful to health
(WHO, 2003).
Available experience and scientific evidence show that the regular practice of appropriate
physical activity and sports provides people, of all ages and conditions, with wide range of physical,
social and mental health benefits. Regular practice of physical activity and healthy sports interacts
positively with strategies to improve diet, discourage the use of tobacco and other unhealthy habits, helps
reduce violence, enhances functional capacity and promotes social interaction and integration.
Preservation of the mechanical output of the skeletal muscle seems to be dependent on their
function and gravitational loading. To understand effect of regular physical activity, scientists propose to
study the opposite - deconditioning as an effect of spaceflight (Antonutto et al. 1999) or ground-based
models such as immobilisation (Sargeant et al. 1977), lower limb suspension (Berg et al. 1991), or bed
rest (De Boer et al. 2008) simulating the effects of microgravity. The postural muscles such as the plantar
flexors and knee extensors appear to be the most affected (De Boer et al. 2008; Di Prampero and Narici
2003). The most common and apparent adaptation of skeletal muscle with removal of weight bearing
contributing to the decreased mechanical output is a loss of muscle mass reflected in a decreased muscle
size (LeBlanc et al. 1995; Akima et al. 2000), muscle strength (Berg et al. 1991; Antonutto et al. 1999),
reduced neural activation (Antonutto et al. 1999), muscle fibre specific tension (Larsson et al. 1996;
Trappe et al. 2004), muscle tone (Pišot et al., 2008), and, recently, the stiffness of the in-series tendon
(Kubo et al. 2000; Maganaris et al. 2006) have been shown to be associated with the loss of muscle
function after disuse and unloading.
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EQOL (2009) 19-24
But there is reasonable doubt in generalising effects of simulating microgravity into effects of
regular sport activity. Deconditioning after microgravity exposure is acute, exposure to deconditioning
lasts for about 14 - 90 days and as such it is not the best model to understand the long-lasting effect of
regular sport activity.
Our goal was to evaluate the age and sport activity type effect of postural and non postural
skeletal muscles’ contraction time in master athletes and non athletes. Master athletes continue their
sportive career throughout life, often adhering to training regimes of 20 hours and more per week.
Therefore, master athletes provide a unique opportunity to study the question pointed out (Rittweger et al.
2004).
Methods
MASTER ATHLETES: Altogether of 170 master athletes (99 males) were measured on 16th European
Veterans Athletics’ Championships Stadia in Ljubljana/Slovenia. Table 1 summarises the number of
participants per gender, age and sport type. Three groups of sport activity type were defined as FAST
(sprinting events up to 800 meters, jumping events, throwing events) and ENDURANCE (running events
longer than 1500m, walking events), while the third group used as control group of non athletes.
NON ATHLETES: The group of 40 non athletes (27 males) were chosen from our previous study
(Šimunič et al. 2005) and 11 (5 males) from yet unpublished data. Their age range was from 35 to 82.
Table 1: Number of participants per age, and sport activity type.
35 - 44
45 - 54
55 - 64
over 65
years
years
years
years
FAST MASTER ATHLETES
27
29
21
29
ENDURANCE MASTER ATHLETES
18
16
15
15
NON ATHLETES
19
12
10
10
FAST: sprint, jump and throw events; ENDURANCE: middle and long distance events; NON
ATHLETES: sedentary population.
CONTRACTION TIME MEASUREMENTS: Tensiomyographic data was assessed using TMG-
ZD1 device (Furlan & Co., Slovenia) on two skeletal muscles. The measurement point in biceps femoris
muscle was defined at the midpoint of the line between the fibula head and the ischial tuberosity, while in
the vastus lateralis was defined at the lower third of its length from the distal insertion. Supramaximal
single bipolar twitch stimulation (pulse width 1 ms) was applied to each muscle, separately. Self adhesive
electrodes (Pals, AxelGaard Co., USA) were placed directly on the muscle belly: cathode was placed 5
centimetres distally, while anode 5 centimetres proximally from the measurement point. Knee joint was
set appropriately for each muscle: for vastus lateralis was set in 30 degrees flexion (participant lying on
his back) and for biceps femoris in 5 degrees flexion (participant lying on his front). Amplitude of the
electrical stimulation was gradually increased to get maximal response where two maximal responses
were saved on the computer for further analysis.
DATA PROCESSING: From two maximal responses amplitude a contraction time was
calculated from 10% to 90% of maximal amplitude contraction. An average value was taken into account
for further analysis. Furthermore, contraction time for each muscle was pilled in twelve different groups:
age (4) x sport activity type (3), as presented in table 1.
STATISTICAL ANALYSIS: Statistical analyses were done using SPSS software version 12.0.
All values are presented as mean values with standard deviation. The age and sport activity type effect
were tested with two-way ANOVA design. Post-hoc Tukey test was used for establishing age effect in
multiple comparisons. The level of significance was set at * P < 0.05, and # P < 0.001.
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Results
Table 2 presents the statistical output for each factor’s main effect and between factor interactions:
-
BICEPS FEMORIS: We observed significant age effect (P < 0.001) and ssignificant sport
activity effect (P < 0.001). However, there was also significant interaction age * sport activity
effect (P = 0.044).
-
VASTUS LATERALIS: Age effect was significant (P = 0.032) but the deterioration was
much lower than in biceps femoris. Interestingly, the sport activity type effect was not
significant (P = 0.526). However, we found significant interaction age * sport activity type
effect (P = 0.045).
Post hoc analysis revealed that biceps femoris contraction time deteriorated significantly in age
group 55 - 64 years for 28% (P < 0.001), and in age group > 65 years for 49% (P < 0.001). Vastus
lateralis contraction time deteriorated significantly in age group > 65 years for 8% (P = 0.012). In Figure
1 contraction time of biceps femoris and vastus lateralis is presented for all three sport activity types and
four age groups. It is evident that biceps femoris changes are larger than in vastus lateralis. Furthermore,
FAST athletes have shorter biceps femoris contraction time at age of 35 - 44 years than ENDURANCE
athletes, while at age of > 65 the differences are no longer present. Contraction time in non athletes at age
of 35 - 44 years have values in the middle regarding to FAST and ENDURANCE athletes. But later on
contraction time consistently increase with age to the values higher than in both groups of athletes. In
vastus lateralis muscle we can establish significantly shorter contraction time at age of 35-44 years but the
effect of sport activity is not significant.
The interaction effect of age * sport was significant in both observed muscles (Table 2). In biceps
femoris contraction time increase has larger slope in non athletes, than in FAST athletes, and least in
ENDURANCE athletes. While in vastus lateralis age and sport effects were much smaller but anyway we
could confirm that larger slope was observed in FAST athletes, than in ENDURANCE athletes and least
in non athletes.
Table 2: Factors’ main effect with their interactions on contraction times of biceps femoris and vastus
lateralis.
Factors /
Biceps
Vastus
interactions
femoris
lateralis
F / P
F / P
Age
31.774 / 0.000 #
3.007 / 0.032 *
Sport
11.913 / 0.000 #
0.644 / 0.526
Age * Sport
2.210 / 0.044 *
2.199 / 0.045 *
* P < 0.05; # P < 0.001
Conclusions
The major part of this study was performed on master elite athletes competing on European athletics
championships 2008. The selection of athletes was done through advertisement in a brochure before the
competition, posters at the stadium and oral invitations. Each athlete went through a registration phase for
basic personal data collection, information on training habits, personal records, nutrition habits, and
health status. Second part of this study was summed from our previous publication (Šimunič et al. 2005)
where we presented muscle deterioration in non athletes. The data for the third part of this study have not
been published yet.
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EQOL (2009) 19-24
Figure 1: Contraction time of biceps femoris (upper graph/table) and vastus lateralis (lower graph/table)
with age and sport type.
FAST MA
ENDURANCE MA
NON ATHLETES
60
50
40
30
20
35-44
45-54
55-64
> 65
Age / years
Biceps femoris Tc ± SE / ms
35 - 44 years
45 - 54 years
55 - 64 years
> 65 years
FAST MA
26.5 ± 1.6
30.9 ± 9.4
37.3 ± 5.1
44.1 ± 9.8
ENDURANCE MA
34.4 ± 2.2
39.4 ± 2.5
41.6 ± 2.3
44.5 ± 2.5
NON ATHLETES
30.9 ± 1.9
32.9 ± 2.4
43.9 ± 2.7
54.2 ± 2.7
FAST MA
ENDURANCE MA
NON ATHLETES
60
50
40
30
20
35-44
45-54
55-64
> 65
Age / years
Vastus lateralis Tc ± SD / ms
35 - 44 years
45 - 54 years
55 - 64 years
> 65 years
FAST males
23.2 ± 0.7
25.0 ± 0.7
25.5 ± 1.0
28.1 ± 0.7
FAST females
25.6 ± 1.0
24.0 ± 1.2
26.7 ± 1.1
27.5 ± 1.2
ENDURANCE males
26.3 ± 0.9
26.3 ± 1.1
24.7 ± 1.2
26.5 ± 1.9
FAST - sprint, jump and throw events; ENDURANCE: middle and long distance events; NON
ATHLETES: sedentary population.
We have found that biceps femoris muscle (non postural muscle) deteriorate in much greater
scale than vastus lateralis muscle (postural muscle) with age. Furthermore, we observed sport activity
type effect in biceps femoris, while not in vastus lateralis. Sprinters and jumpers have shorter contraction
time in both muscles at age of 35 - 44 years and ageing brings them to the values equal of endurance
athletes. This finding confirms that endurance type training could be useful at preserving physical fitness
at old age. We could confirm also age * sport activity type interaction effect was significant in both
muscles. Postural muscle deteriorates with age the most in fast athletes but we have to consider shorter
contraction times in baseline at age of 35 - 44 years. In non postural muscle bigger age deterioration is in
non athletes, even though their baseline contraction time is not the shortest.
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We evaluated this with contraction time derived from mechanical response of muscle belly, using
Tensiomyography. Tensiomyography was presented few times as a useful tool for non-invasive and
selective detection of skeletal muscle contractile properties (Dahmane et al. 2000, 2005; Pišot et al. 2008).
Authors explained contraction time as intrinsic contractile properties of muscle belly detected on the
muscle belly and not on a distal limb (torque mechanical response). Such approach prevents integration of
tendon properties, joint mechanics, and surrounding tissue effect on mechanical response deformation.
There is an undoubted loss of skeletal muscle mass and strength with age in humans. The atrophy
of skeletal muscle with age appears to be due to a decline in the hormonal and nutritional status
(combined with changes in many other systems), reduced exercise and muscle use, and a loss of muscle
innervation. It is not clear whether the age related changes in muscle activity result in a lack of production
of trophic factors by the muscle that are required to maintain healthy synaptic connections between the
muscle and the nerve. Alternatively, the neurones themselves may manifest some age-related defect,
resulting in an inability to correctly function and innervate the muscles. It would seem that the balance
between these two scenarios remains to be unresolved.
Runge et al. (2004) has found that postural calf muscle anatomical cross sectional area does not
correlate with age, neither in males nor females. Furthermore, the same authors found significant negative
correlation in jumping power with age, for both genders. Functional but not geometrical loss within
skeletal muscle indicated deterioration in skeletal muscle composition or neural drive. Lexell et al. (1988)
showed that the ageing atrophy of the muscle begins around 25 years of age and thereafter accelerates.
This is caused mainly by a loss of fibers, with no predominant effect on any fiber type, and to a lesser
extent by a reduction in fiber size, mostly of type
2 fibers. Since our data was collected using
transcutaneous electrical stimulation and not by voluntary contractions, we suggest that more neural drive
than fibre type deterioration affect muscle function.
Acknowledgements
The authors are grateful to master athletes competing at the European veterans’ athletics gala in
Ljubljana/Slovenia who were willing to participate in all our studies. We are thankful to the organisers for
their flexibility and big help at setting up our equipment and to all the students for their help at
recruitment of the master athletes.
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